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How To Build New Blood Vessels, Regenerate Muscle Tissue Fibers, Increase New Cell Growth & More With Something Called “TB-500”.

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Last month, in the article “How To Use BPC-157: A Complete Dummies Guide To Healing The Body Like Wolverine“, I introduced the little-known concept of using BPC-157 peptide self-injections and oral BPC-157 peptide consumption (currently completely legal and not banned by sporting organizations) for everything from rapidly healing leaky gut to fixing tendon, ligament and muscle injuries.

In that article, you learned how to order BPC-157, how to “reconstitute” BPC-157 to make it biologically active, how to inject BPC-157 either intramuscularly or subcutaneously, how to take it orally, and BPC-157 research and side effects. I'd highly recommend you review that article prior to reading this one.

Since writing about BPC-157, I've taken an even deeper dive into the underground world of peptides, and found another similar peptide to BPC-157: something called “TB-500“, also known as Thymosin Beta 4.

TB-500 is a synthetic peptide that has been directly linked to impressive extremely enhanced recovery times for muscle and more. It plays a vital role in building new blood vessels, new muscle tissue fibers, new cell migration and new blood cell reproduction.

TB-500 acts a bit differently (and in some cases, more effectively and quickly) than BPC-157 and in today's article, you're going to discover exactly what TB-500 is, how it works, how to use it, and any and all pre-cautions and side effects you should know about.

A warning: unlike BPC-157, TB-500 is absolutely, 100% banned by WADA and most other global sporting organization both in-competition and out-of-competition. You should NOT use this if you are competing in any such sanctioned sport as it definitely falls under the banned category of “Peptide Hormones, Growth Factors, Related Substances and Mimetics (S2)”.


What is TB-500?

TB-500, or Thymosin Beta 4, is a peptide that naturally occurs in the human body and in animal bodies, and, since it is mostly sold for research purposes and veterinary use, is typically implemented in clinical trials in horses.

Thymosins were discovered in the mid 1960’s, when Allan Goldstein from the Laboratory of Abraham White at the Albert Einstein College of Medicine in New York studied the role of the thymus in development of the vertebrate immune system. Since then, Dr. Goldstein founded a company that creates thymosin alpha 1 for the purpose of increasing immune cell activity, and thymosin beta 4 (TB-500) to promote wound repair and healing.

TB-500 has been used extensively for race horses to prevent adhesions from forming, although it is not a prescription veterinary drug. It’s an injectable peptide with limited human use. Mostly, it’s limited to humans who like to experiment, although reports of human use of thymosin dates back as far as 1974 – when a young girl became the first person to receive injections of thymosin because she was diagnosed without a functioning thymus gland.

TB-500 has been shown to offer many of the same effects of growth hormone (GH), including:

-Increasing strength through muscle growth
-Improving endurance
-Speeding up the healing process for wounds
-Reducing acute/chronic pain and/or inflammation
-Increasing flexibility
-Increasing hair growth

As you learned in the previous article on BPC-157, peptides are two or more amino acids linked together in a chain, and TB-500 is a simply a short peptide fragment of an actual hormone – a hormone called “Thymosin Beta-4”.

TB-500 and Thymosin Beta-4 are not exactly the same, although you’ll often see the two names used interchangeably in the peptide world (AKA broscience bodybuilding forums).  It’s much harder to get your hands on true Thymosin Beta-4, whether for research use, equine enhancement, athletic performance enhancement or bodybuilding. But TB-500’s peptide sequence shares most of the properties of Thymosin Beta-4, and it’s more economical to produce, thus easier to find.

Just in case you care about the specifics or you really, really miss high school chemistry class, you can dig into the molecular formula of TB-500 below. It is C212H350N56O78S and has an amino acid sequence of the following

Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser.

TB-500

So, how does this stuff work?

The main functionality of TB500 hinges on the ability to upregulate cell building proteins such as actin, which is a protein that forms (together with myosin) the contractile filaments of muscle cells, and is also involved in motion and metabolism in many other types of cells.. Upregulation of actin allows TB500 to promote healing, cell growth, cell migration and cell proliferation. This not only helps build new blood vessel pathways but also upregulates the type of “good” inflammation that is directly correlated with faster wound healing.

Due to its molecular structure and low molecular weight, TB-500 is very versatile, mobile and possesses the ability to travel long distances through tissues. This means that when targeting injured areas (chronic or acute), TB-500 has the ability to circulate through the body and “find” those areas of injury in order to enhance the healing or growth process. Many users have also noted the added benefits of improved flexibility, reduced inflammation in tendons, re-growth of lost hair, and darkening of grayed hair.

Currently, studies are also being conducted on the ability of TB-500 to heal damaged heart tissue, including “athlete's heart”, also known as ventricular hypertrophy.

In the past, TB-500 has been used extensively for race horses to prevent muscular adhesions and injuries from forming, although it is not a prescription veterinary drug, and MedivetEquine reports that TB-500 offers a variety of benefits to all mammals, regardless of size, including:

In the majority of humans who experiment with TB-500 injections, TB-500 is most often used for acute injuries or wounds that would heal slow (when you need them to heal fast), or for more chronic injuries where the healing doesn’t seem to be happening.


Where to Get TB-500

You can find TB-500 (often sold under the name Thymosin Beta 4) on several peptide supplier websites. These websites can only legally sell the peptides for research purposes. For example:

When you get your TB-500, it will come in a powder form. Just like BPC-157, you will need to “reconstitute” it by adding bacteriostatic water. Go back and read my article on BPC-157 to get access to a peptide calculator that will help you with the mixing/dosage math. Once your TB-500 is properly mixed, you then draw the dose into an insulin syringe, and inject it intramuscularly, subcutaneously, or intravenously (according to your preference).

Supplies needed for TB-500 injection:

IMG_4195

IMG_4192

IMG_4194

Steps for Mixing TB-500:

  1. Take the lids off both your bacteriostatic water and TB-500 containers.
  2. Rub the alcohol swab along the rubber stopper on the TB-500 container, and the top of the bacteriostatic water vial and allow each to air dry.
  3. Fill the insulin syringe with bacteriostatic water and slowly and carefully inject the syringe of water into the 5mg bottle of TB-500 – being careful to spray the water onto the sides of the container rather than directly into the powder. If you do this three times, you will nearly fill the 5mg bottle of TB-500.
  4. Your TB-500 is now reconstituted. You will want to keep it in the refrigerator or freezer to preserve it until you’re ready to use it.

Do not let your mixed TB-500 sit at room temperature or in the light or it will go bad. To ensure the highest quality, it needs to be kept out of direct light and at refrigerated or frozen temperatures.

When drawing TB-500 into syringes for injection, turn the bottle upside down slowly and draw the liquid into the syringe smoothly and slowly. When injecting, do so with a smooth, slow motion as well. Peptides are fragile and too much mixing and jostling of the container can damage or reduce the effectiveness of your product.


How to Inject TB-500

TB-500 can be delivered through subcutaneous, intramuscular, or intravenous injections.

Before using any of these methods, use a fresh alcohol swab to clean the rubber stopper of your TB-500 bottle and the area of injection. This will minimize your risks for bacteria and contamination.

Subcutaneous injections – you simply pinch an area of skin and push the needle through it. Tip: you should inject an area of your body near your actual injury.

Intramuscular injections – these are harder than subcutaneous injections as instead of simply inserting the needle under the skin, the needle must be “stabbed” into the muscle as close to the area you are experiencing pain or injury as possible.

Intravenous injection – the needle is inserted directly into the vein, usually on the wrist, elbow, or back of the hand. This one is pretty tough, and I don't recommend unless you have medical supervision or really know what you're doing.

After injecting TB-500, massage the area of injection for about a minute to help work the TB-500 into the tissues and improve the absorption and effectiveness.

The most common dose of TB-500/Thymosin Beta 4 is based on your body weight. For a 200-pound person, the dosage recommendation is about 7.66mg per week for six weeks. This is derived by calculating the human dose based on the recommended dosage of TB-500 for greyhounds (5 mg) according to MediVet (the TB-500 manufacturer).

tb500dose

At the risk of sending you down a broscience rabbit hole, here's a link to a bodybuilding forum discussion that delves into TB-500 dosage in more detail, along with some practical user experiences.


Side Effects and Risks Of TB-500

You should know that there have been some conflicting research studies about this stuff when it comes to cancer. Some studies suggest that it may contribute to the growth of existing cancer cells, while further data suggests it may inhibit cancer.

Doctors have noticed cancer patients have a higher amount of Thymosin in the affected tissues than other people. So in the early stages of research, doctors assumed that this meant Thymosin may cause cancer. After more research was conducted, it was discovered that the main action of Thymosin Beta 4 was to produce new white blood cells – so its presence in the body in the areas affected by cancer was likely not a cause of the cancer, but instead, a matter of “showing up” in the body where cancer lived to help the body mount an immune system response.

But like most peptides on the market, TB-500 has limited long term studies involving human use. Although I haven't personally used TB-500 (I can't, since I compete in WADA sanctioned sports like triathlon and obstacle course racing), from what I’ve seen and heard from bodybuilders and athletes using the peptide, the primary side effect is a temporary sense of lethargy. Also, some people report getting a head rush when injecting TB-500, but report this head rush goes away a few minutes after injecting.


Summary

Here's the deal: peptides are the wild, wild west of supplementation.

Many are illegal for sanctioned sporting purposes, so do your research (I recommend GlobalDro.com for this).

Many do not have long term safety studies in humans, but decent track records in animals.

Proceed at your own risk.

But when it comes to healing injuries fast, healing the gut, growing new muscle faster, or getting any of the other benefits I mentioned in this article, it may be worth looking into peptides such as TB-500 or BPC-157.

Do you have questions or comments about TB-500? Have you used it yourself? Let me know in the comments below. I'd love to hear about your experience, and any tips you have for peptide use.

The information in this article is provided as an information resource only, and is not to be used or relied on for any diagnostic or treatment purposes. This information is not intended to be patient education, does not create any patient-physician relationship, and should not be used as a substitute for professional diagnosis and treatment.

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87 thoughts on “How To Build New Blood Vessels, Regenerate Muscle Tissue Fibers, Increase New Cell Growth & More With Something Called “TB-500”.

  1. BPC 157 for healing gut also has a good potential when taking as enema technically . For healing leaky gut etc . So that may be way to try it , should be very potent that way and has been done before. Did you have anny luck shuttint down leaky gut etc with oral administration Ben ? Lactulose / mannitol testing before after ? BLP?

  2. Hi Ben, I am interested that you mention one of the effects as reduced inflammation in tendons. Have you come across anything to eliminate Dupuytren’s constricture, which is a disease of tendons in the hand, without surgery?

    I’m always interested in your research. Many Thanks, Pete

  3. Ben, are there other ways to heal faster/ recover faster from training, that dont involve injecting yourself? lol

  4. hi..had three torn muscles between shoulder and bicep..all needed surgery for re attachment.. did a 20 day round of 157.. believed it helped..looking for a suggestion..another round or do you think the TB500 may be better? im at 7weeks of a 6-8month healing process. Thank for these articles and any insights-recommendations you may have.

  5. Hi..i had 3 major muscle tears(complete)..2 in shoulder and 1in bicep..they all needed surgery for reattachment.. ive done a 20 day round with 157 and believed it helped..im at 7 weeks post op and have about 5 month’s of recovery still to go.. thinking another round of 157..wondering if the TB500 might be a better option or stay with 157? As an athlete and top Spartan racer,im looking to speed up process by any %. Any thoughts/opinions or recommendations are greatly appreciated! Thanks for your help and this info :)

    1. Spartan does not test.. And i missed this year due to this injury.. Judt want to know if the TB500 would be a good choice given my injury.. Will be 12 months before any racing.. Might not do Spartan either.. Just local stuff.. Thanks

    1. And drinking soy increases your risk of endometrial hyperplasia.

      The same arguments are seen with insulin, insulin growth factor (IGF), HGH, and anything that increases over expression and up regulation of androgenic receptors.

      I think it’s all about what your goals are as anything that isn’t organic and 100% natural is technically foreign to your body and has its side effects

    2. The research goes back and forth. I highly suspect that in a case of mitochondrial integrity – such as discussed in my recent podcast with Dr. David Minkoff on what really causes cancer – it is a nonissue. But that is just a hypothesis.

  6. Hi Ben, I have been using TB-500 for minor injury repair assistance for more than 2 years. I have found it to be extremely effective for minor strains to calves, hamstring, shoulder etc. Luckily I have not had to try it for any major injuries. When I first used it I was blown away by how effectively it worked – even to the point that I began to doubt the seriousness of the original injury. When injured I dose at 5mg per week for four weeks then take at least four weeks break. The only side affect I have noticed is a little light headed feeling which passes pretty quickly. I am in my late 40’s and I train hard. TB-500 allows me to train through minor injuries which is great. Love your work

  7. I have an upcoming surgery and their will be a very long incision basically from my knee to ankle on both legs. Do you feel this would help with healing the would and potential scar tissue. Is TB 500 or BPC a better choice or both??

  8. I have taken BPC-157 in conjunction with TB-500 after reading about someone’s experience. I used the BPC-157 at an injury/inflammation site in my shoulder. I have a pain that came out of nowhere and has prevented me from doing bench presses mainly, and shoulder presses. I also got pain when I did external rotation of my shoulder. The BPC-157 gave me good results at 250 mcg twice daily intramuscularly. The pain is not completely gone but it has definitely lessened in severity. I don’t get any pain with a reverse grip press so I have been doing those with light weight and I can now do shoulder presses. BPC-157 really blew me away on how quickly it improved my gut status. For me it only took 4 days of orally dosing with 250 mcg. So I did both the oral and intramuscular daily for a month. Two weeks into the BPC-157 I ordered TB-500 and did 1mg per week subq in my thigh because I didn’t know about injecting intramuscularly at the injury site.

    With the TB-500 it seems that pain was reduced even more in my shoulder and it appears that I recovered much faster from my workouts. I took the TB-500 on rest days. I have two more 1mg doses of TB-500 and I am going to site inject intramuscularly to the shoulder to see what happens. Then I will stop taking both for a month to see how things work out. Hopefully I won’t need them again.

    Thanks for the info Ben!

  9. I’m always interested in learning about better supplements for my health. I’ve heard a lot of good things about peptides, but this was the first time I’ve read about TB-500 in particular. It sounds like it can have a major impact on helping you recover from injuries, which is a huge deal in today’s world. I may need to look into it some more before actually buying it, but thank you so much for taking the time to explain it!

  10. Ben,

    I’ve recently been diagnosed with osteoarthritis in my right knee, with significant cartilage damage. Would either TB-500 or BPC-157 be worth trying in this situation?

  11. Peptides seem different than anabolics /hgh in the fact that (i’m asking) they don’t shut down or disregulate any of your bodies natural productions /processes or make you feel “weak” it different after stopping them correct?

  12. Ok Ben ordered the stuff as you suggested. 3 bottles. Going to give it a shot on my elbows which have a bit of tendinitis from doing lots of ring and bar work.

    Do I try and inject it right into the tendon?

    How long does the mix last?

    Also strained my back a bit would this work for that as well? Inject it into the lower back muscle?

    Any advice would be aprciated. I do believe you used it on your elbow tendinitis as well so I would be interested on your take of how to do the injections there.

    Thanks.

      1. Ok Ben. Thanks. Started the TB 500 for my elbows. Got the 5mg of TB 500 and reconstituted it with 3 cc/ml. of water (3 syringes full) Just about filled the file. Now based on injecting just under .1 cc/ml or just under 10 (8) units for a dose of around 250. How long did that vial last you? Seems like there is a lot left and the amount injected is small. Is my dose and math right?

    1. Should I use TB-500 or BPC-157 for an Achilles. I recently tore it and was wondering if any of these products could speed up my recovery?

  13. Hey Ben,

    Is there any reason you didn’t list Blue Sky Peptides with your sources for this one?

    Wondering if you discovered something about quality since the previous BP-157 post.

    Thanks!

  14. Hey Ben

    Can you pre-fill syringes with tb500 and put in the freezer until use. Do you have experience freezing this peptide and using it later? I heard once tb-500 has been reconstituted, you have to use it up in 8 days before it goes bad!

  15. Hey ben , great article.

    Would combining BPC-157 with TB-500 be same as combining it with MK-677 or any other GH increaser for recovery ? Or is the BPC-157 + TB-500 combo the way to go ?

    Thanks

  16. There is a site in Australia selling TB-500 reconstituted in 15ml vials. They say to go with 25 units daily for loading. This would mean one month from the same vile. Are you saying that it would be”bad” after 8 days?

    1. I can ONLY vouch for the TB-500 source I have listed here (peptides warehouse)…and the loading scheme I recommend above. That is *all* I ill vouch for! Keep any you don't want going bad in the freezer btw.

  17. Ben,

    My wife is an elite athlete who had hip surgery for a torn labrum with impingement. Recovery is super slow and testing is not a current concern… Any suggestions for site and frequency of injection?

  18. This is amazing! The use of the TB-500 and Thymosin Beta 4 for humans are limited and it can increase muscle growth, improve endurance and most of all can speed up the healing process of wounds. Essentially, peptides are small proteins that can help to boost the naturally occurring hormones within your body, to help you achieve better results.

  19. Hi Ben and thanks for all the great information! Just wondering if you see any reason that I shouldn’t start a TB500 cycle almost immediately after a BPC157 cycle. It’s only been a few days after I’ve finished my BPC157 (with some “success”) but I’m anxious to being the TB500. Thoughts? Thanks!

  20. Can I take TB500 with SARMS? If so, which stack would you recommend for lean muscle and strength? I also have some tendon pain on the inside of my elbow, thinking TB500 could help.

    Thanks

  21. Hi Ben!

    Have you had any personal experience or know anyone who has used IGF-1 LR3 & DES?

    Thank you for all the informative work you share!

    Regards.

  22. Hey Ben,
    What is the equation you would used to determine how much TB 500 to use per kg? Wondering how many 5ml vials I need to purchase to treat bilateral anterior shoulder pain.
    Thx, Jb

  23. Hi Ben,
    This may be an odd question. But would this be good to inject or spray on those that have undergone hair transplants in the first month of surgery. Fue microscars. I see you mention it improves hair growth and blood flow. both which basically what rogaine claims to do. Surely this would benefit new transplanted hair follicles in taking and holding? Interested in your thoughts in the matter.

    Thanks,
    Dom

  24. Ben, just to make sure I’m understanding the maths correctly and not going crazy on the dose. Based upon the recommended dose of 7.66mg / week, it would be expected to use up 1.5 vials of this stuff per week for a 200 pound person?

    Alos, based on what you read, do you have any thoughts about how best to do this? One shot once a week or a set number of days apart?

  25. Ben, i remember you saying you had sprained your ankle during one of the spartan and you used this as a recovery. How exactly did you inject into the ankle?

  26. Hello, I have started taking bpc157 and tb500 last week to help with tendinitis of the patellar and an SI injury. Wasn’t expecting much for the SI but I can say something is definitely working. Both are feeling much better and I’m only just over a week in.

    I was kind of scared because I ran across some threads that said TB500 leads to cancer or promotion of benign tumors…most of these were at least 4-5 years old though and it seems there are countless logs online all with good experiences. Nonetheless I was still worried so I did some more research and came across a pharmaceutical company in the US doing clinical trials for thymosin beta 4 to help with dry eye syndrome. I have attached some links. This makes me feel much safer but if you have any more insight I’d really appreciate it.

    http://www.prnewswire.com/news-releases/regenerx-…

    http://www.regentreellc.com/?c=tbeta4

  27. As BPC 157 and TB500 seem to have different mechanisms of actions could it make sense to take them together or are there any contraindications you’re aware of?

  28. Hey Ben! Thank you for all of your awesome content. I am wondering if you are familiar with Medivet Equine’s TB-1000 which they market to be twice as concentrated and potent as TB-500? I can find no clinical research to support this claim, and am wondering your thoughts/opinion. Thanks so much!

  29. Ben, what about using this or bpc 157 for the SI joint? Mine has been acting up for years. A lot if cracking and popping. It’s definitely a weak link. But it just seems like a scarey spot to go IM.

    1. I am not a doctor and this is not to be taken, interpreted or construed as medical advice. Please talk with a licensed medical professional about this. These are just my own personal thoughts and not a prescription or a diagnosis or any form of health care whatsoever.

      If I were you, for the SI joint I would do this exact program: http://www.RunWithNoPain.com – that's why i wrote it!

  30. Hi Ben

    Ive been struggling with my left brachialis and so after listening to a podcast in which you mentioned BPC-157 I tried it out. I was hoping for a miracle cure, which I did not get. However, I believe it certainly helped a lot. I am considering a second round. But the peptide rabbit hole is certainly interesting and I am most keen to try some others. But I would like to see if you have some advise for me on another topic. After having listened to your podcast with Dr Gains I thought that either you or Dr Gains may be able to at least point me in the right direction. My wife suffers from a condition called Pelvic Vein Congestion which causes her a lot of pain. From what I understand, she has a seemingly unnatural mass of veins around her uterus which may also suffer from a “valve” type problem in which blood can potentially run in the opposite direction and pool in locations which causes pain. She has been to doctors which offer invasive solutions with unattractive success rates. I have been searching for other cures, but the only thing that I found (supplement with Diosmin and Hisperidan) had some psychological effects. Any thoughts?

    1. I am not a doctor and this is not to be taken, interpreted or construed as medical advice. Please talk with a licensed medical professional about this. These are just my own personal thoughts and not a prescription or a diagnosis or any form of health care whatsoever. In terms of doctors, here are a few directories that may help you find a good functional medicine or naturopathic practitioner in your area:

      • HealthProfs.com
      • FunctionalMedicine.org
      • Naturopathic.org
      • PrimalDocs.com
      • PaleoPhysiciansNetwork.com

      That will ensure you find someone who I would likely vouch for!

      Finally, I'd be happy to help you via a personal one-on-one consult. Just go to http://greenfieldfitnesssystems.com/product/ben-g… and then choose a 20 or 60 minute consult, whichever you'd prefer. I can schedule ASAP after you get that.

  31. I notice unlike BPC-157, TB-500 usually doesn’t list ligaments/tendons in the list of benefits (you only mention reducing tendon inflammation). Would it be accurate to say BPC-157 is better for joints/ligaments/tendon/cartilage while TB-500 is better for more vascular muscles/skin/etc?

    I’m curious why you say TB-500 is stronger, the studies showing BPC-157 healing severed ligaments/tendons seems way more radical and impressive than TB-500’s merely lowering tendon inflammation, and for most people connective tissue injuries are the much more problematic issue.

    >TB-500 is a synthetic peptide that has been directly linked to impressive extremely enhanced recovery times for muscle and more. It plays a vital role in building new blood vessels, new muscle tissue fibers, new cell migration and new blood cell reproduction.

  32. Wondering which would be more affective for ligament and tendon tears TB500 or BP175 I’m currently dealing with a Scapholunate ligament tear.

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